Differences in Na+,K+-pump function between spontaneously hypertensive and normotensive rats are not attributable to intrinsic biochemical differences in the purified Na+,K+-ATPase enzyme.
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Excludes intrinsic Na+,K+-ATPase defects in SHR hypertension models; leaves open regulatory or extrinsic factors for targeted investigation.
Na+,K+-ATPase (EC 3.6.1.3) was purified to homogeneity from the kidney of spontaneously hypertensive rats (SHR), and their normotensive control rats (Wistar Kyoto rats; WKY. Purity of the enzyme was confirmed by sodium dodecyl sulfate (SDS)polyacrylamide gel electrophoresis, in combination with immunoblotting using anti-dog Na+,K+-ATPase antibody. The enzyme purified from SHR and WKY did not differ in respect to the molecular weight, subunit composition, specific activity, ouabain sensitivity, requirements for Na+ and K+ (and also for Mg2+ and Ca2+), and to the extent of heat denaturation. The results indicate that the difference in Na+,K+-pump so far reported between SHR and WKY is not attributable to any difference in biochemical properties of the purified Na+,K+-ATPase.
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Ohta et al. (1984) studied this question.
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