What question did this study set out to answer?

To characterize the positive upstream regulatory site and determine how it interacts with an adjacent negative regulatory site to control oxygen-dependent CYC7 expression in Saccharomyces cerevisiae.

June 1, 1987Molecular and Cellular BiologyOpen Access

Elements Involved in Oxygen Regulation of the Saccharomyces Cerevisiae CYC7 Gene

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Authors

RZRichard S. ZitomerUniversity at Albany, State University of New York JSJoan W. SellersHarvard University DMDavid W. McCarterUniversity at Albany, State University of New York

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Implication

Mutational analysis reveals dual upstream regulatory sites control oxygen-dependent CYC7 expression in yeast, indicating coordinated repression under anaerobic conditions.

Key Points

To characterize the positive upstream regulatory site and determine how it interacts with an adjacent negative regulatory site to control oxygen-dependent CYC7 expression in Saccharomyces cerevisiae.
Conducted mutational analyses targeting the positive regulatory sequence situated at -240 base pairs upstream of the CYC7 coding region.
Evaluated gene expression across wild-type strains, CYP1-16 mutant lines, and deletion constructs lacking either the positive (-240) or negative (-300) regulatory sites under aerobic and anaerobic growth conditions.
Demonstrated that aerobic induction and CYP1-16-mediated transcriptional enhancement are driven through the positive site, specifically requiring a 9-base-pair tandem direct repeat.
Observed that genes harboring only the negative site showed anaerobic stimulation, whereas the intact configuration of positive and negative sites combined to effectively repress anaerobic transcription.

Cite This Study

Zitomer et al. (1987) studied this question.

synapsesocial.com/papers/6a8a8558ec2e105e8ac14fe9 https://doi.org/10.1128/mcb.7.6.2212-2220.1987

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Implication

Mutational analysis reveals dual upstream regulatory sites control oxygen-dependent CYC7 expression in yeast, indicating coordinated repression under anaerobic conditions.

Elements Involved in Oxygen Regulation of the Saccharomyces Cerevisiae CYC7 Gene

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Implication

Key Points

Cite This Study

Elements Involved in Oxygen Regulation of the Saccharomyces Cerevisiae CYC7 Gene

Discussion

Member takes

Implication

Key Points

Cite This Study

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