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March 11, 2015Journal of Biophotonics

Non‐linear imaging and characterization of atherosclerotic arterial tissue using combined SHG and FLIM microscopy

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Authors

RCRiccardo CicchiNational Institute of Optics EBEnrico BariaInstituto de Óptica "Daza de Valdés"CMChristian MatthäusLeibniz Institute of Photonic Technology

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Implication

Laboratory imaging study demonstrates optical discrimination of collagen and lipid plaques in arterial tissue, highlighting the clinical potential of combined SHG-FLIM microscopy.

Key Points

To evaluate combined second-harmonic generation (SHG) and fluorescence lifetime imaging microscopy (FLIM) for differentiating and characterizing atherosclerotic plaque from healthy arterial tissue.
Applied combined SHG and FLIM microscopy to arterial wall tissue samples with atherosclerosis.
Calculated mean fluorescence lifetimes, aspect ratios, and spatial correlation lengths to distinguish structural tissue components.
Plaque depositions exhibited a significantly shorter mean fluorescence lifetime (1260 ± 80 ps) compared to normal arterial walls (1480 ± 100 ps), allowing clear discrimination between lipids and collagen.
Normal arterial walls demonstrated a more anisotropic aspect ratio (0.37 ± 0.02) compared to plaque depositions (0.61 ± 0.02).
Correlation length analysis separated collagen fiber bundles (2.0 ± 0.6 µm) from cholesterol depositions (4.1 ± 0.6 µm).

Cite This Study

Cicchi et al. (2015) studied this question.

synapsesocial.com/papers/6a8a85d11c70e695d8436be6 https://doi.org/10.1002/jbio.201400142

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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

1Laguerre-based method for analysis of time-resolved fluorescence data: application to in-vivo characterization and diagnosis of atherosclerotic lesions2006 · 55 citations
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