What question did this study set out to answer?

To establish a differentiation protocol that guides pluripotent stem cells specifically toward a ventricular cardiomyocyte lineage by regulating Wnt and retinoic acid signalling pathways.

May 6, 2022Stem Cell Research & TherapyOpen Access

Stage-specific regulation of signalling pathways to differentiate pluripotent stem cells to cardiomyocytes with ventricular lineage

View Full Paper

Ask AI

Bookmark

Why the study?

Pluripotent stem cell differentiation into a heterogeneous cardiomyocyte population increases post-transplantation arrhythmia risk, prompting the need for protocols driving a ventricular lineage.

Population

Mouse embryonic stem cells cultured in monolayers or three-dimensional hanging drop method

Comparison

Different treatments acting on Wnt and retinoic acid signalling

Design

In vitro experimental study

Authors

RSRamakanth SatthenapalliUniversity of Otago SLScott LeeDonald Danforth Plant Science Center JPJayanthi Bellae PapannaraoUniversity of Otago

Discussion

Member takes

Implication

May lower post-transplant arrhythmia risk in preclinical PSC models; leaves open human translation and efficacy trials.

Key Points

To establish a differentiation protocol that guides pluripotent stem cells specifically toward a ventricular cardiomyocyte lineage by regulating Wnt and retinoic acid signalling pathways.
Cultured mouse embryonic stem cells using monolayer cultures and three-dimensional hanging drop methods to form embryoid bodies.
Applied stage-specific modulations of Wnt and retinoic acid signalling pathways in combination with ascorbic acid.
Assessed cardiomyocyte-specific differentiation markers across multiple time points using RT-PCR, flow cytometry, and immunofluorescence.
Treatment with Wnt and retinoic acid modulators plus ascorbic acid yielded an immature cardiac population expressing established markers of cardiac differentiation.
Treated cells upregulated ventricular-specific markers while downregulating pro-atrial and pro-sinoatrial markers.
Differentiated embryoid bodies developed into early fetal-like ventricular cardiomyocytes that contracted upon physiological stimulation.

Structured PICO

Population

Mouse embryonic stem cells cultured either in monolayers or three-dimensional hanging drop method to form embryonic bodies (EBs)

Intervention

Treatments acting on Wnt and retinoic acid (RA) signalling pathways and ascorbic acid

Outcome

Cardiomyocyte-specific markers analysed by RT-PCR, flow cytometry and immunofluorescencesurrogate

In vitro regulation of Wnt and retinoic acid signaling pathways directs mouse embryonic stem cells to differentiate into ventricular-specific cardiomyocytes.

Cite This Study

Satthenapalli et al. (2022) studied this question.

synapsesocial.com/papers/6a8a98df5246293882fd9b07 https://doi.org/10.1186/s13287-022-02845-9

View Full Paper

Ask AI

Bookmark

Stage-specific regulation of signalling pathways to differentiate pluripotent stem cells to cardiomyocytes with ventricular lineage

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Stage-specific regulation of signalling pathways to differentiate pluripotent stem cells to cardiomyocytes with ventricular lineage

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider