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September 26, 2025Stem Cell Research & TherapyOpen Access

Specific induction of right ventricular-like cardiomyocytes from human pluripotent stem cells

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Authors

YSYukihiro SaitoOkayama University KNKazufumi NakamuraHeart Failure & Transplant YKYuki KatanosakaKinjo Gakuin University

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Implication

Experimental study demonstrates targeted differentiation of right ventricular cardiomyocytes from human pluripotent stem cells, indicating new opportunities for chamber-specific disease modeling.

Key Points

To establish an efficient differentiation strategy to generate right ventricular-like cardiomyocytes from human pluripotent stem cells and evaluate their distinct phenotypic characteristics.
Modified an established cardiac differentiation protocol (sequential GSK3β and Wnt inhibition) by administering insulin or BMP antagonists during the mesoderm formation phase.
Assessed cardiac progenitor cells for first heart field and second heart field markers, followed by gene expression profiling and functional phenotyping of differentiated cardiomyocytes.
Standard differentiation predominantly yielded first heart field progenitors and left ventricular-like cardiomyocytes, whereas blocking BMP signaling during mesoderm induction shifted progenitor identity toward the second heart field.
Cardiomyocytes derived from second heart field-like progenitors exhibited right ventricular-like gene expression patterns alongside distinct functional phenotypes, showing altered spontaneous contraction rates, Ca2+ transients, and cell size compared to left ventricular-like controls.

Cite This Study

Saito et al. (2025) studied this question.

synapsesocial.com/papers/6a8c2ecd2ff0ab4f91e96911 https://doi.org/10.1186/s13287-025-04656-0

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Implication

Experimental study demonstrates targeted differentiation of right ventricular cardiomyocytes from human pluripotent stem cells, indicating new opportunities for chamber-specific disease modeling.

Specific induction of right ventricular-like cardiomyocytes from human pluripotent stem cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Specific induction of right ventricular-like cardiomyocytes from human pluripotent stem cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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