A Study of Streptococcal Infection in a School Population: Laboratory Methodology

Article1 December 1958A STUDY OF STREPTOCOCCAL INFECTION IN A SCHOOL POPULATION: LABORATORY METHODOLOGYGEORGES WERNER, Ph.D., DAVID CORNFELD, M.D., JOHN P. HUBBARD, M.D., F.A.C.P., GEOFFREY RAKE, M.D., F.A.C.P.GEORGES WERNER, Ph.D.Search for more papers by this author, DAVID CORNFELD, M.D.Search for more papers by this author, JOHN P. HUBBARD, M.D., F.A.C.P.Search for more papers by this author, GEOFFREY RAKE, M.D., F.A.C.P.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-49-6-1320 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptSince the clinical diagnosis of streptococcal infections is necessarily presumptive, epidemiologic studies instituted to detect such infections depend upon the accuracy of laboratory methods used for the isolation of beta-hemolytic streptococci. In this paper the results of comparative studies of various laboratory technics used in culturing and identifying beta-hemolytic streptococci from the throat swabs of Philadelphia school children will be reported. The data were compiled during an epidemiologic study of streptococcal infection conducted since 1955, and described in an accompanying paper.1These technics vary in some details from those recommended in a statement of the American Heart Association,2and therefore...Bibliography1. CornfeldWernerHubbardWeaverBellows DGJPRM: Streptococcal infection in a school population: preliminary report, Ann. Int. Med. 49: 1305, 1958. LinkGoogle Scholar2. Wannamaker LW (a) : A method for culturing beta-hemolytic streptococci from the throat, American Heart Association Pamphlet, New York, New York, 1956. (b) Mackenzie, G. M.: The streptococci, in Diagnostic procedures and reagents, 3rd Ed., 1950, American Public Health Association, New York, p. 49. Google Scholar3. Pike RM: An enrichment broth for isolating hemolytic streptococci from throat swabs, Proc. Soc. Exper. Biol. and Med. 57: 186, 1944. CrossrefGoogle Scholar4. Lancefield RC: Microprecipitin technique for classifying hemolytic streptococci and improved methods for producing antisera, Proc. Soc. Exper. Biol. and Med. 38: 473, 1938. CrossrefGoogle Scholar5. SwiftWilsonLancefield HFATRC: Typing group A hemolytic streptococci by microprecipitin reactions in capillary pipettes, J. Exper. Med. 78: 127, 1943. CrossrefMedlineGoogle Scholar6. RantzRandall LAE: A modification of the technics for determination of the antistreptolysin titer, Proc. Soc. Exper. Biol. and Med. 59: 22, 1945. CrossrefGoogle Scholar7. StreitfeldSaslawDoff MMMSSD: Group A beta-hemolytic streptococcus and rheumatic fever in Miami, Florida, Pub. Health Rep. 71: 745 (Aug.) 1956. CrossrefMedlineGoogle Scholar8. : Problems in determining the bacterial flora of the pharynx, Proc. Soc. Exper. Biol. and Med. 69: 45, 1948. CrossrefMedlineGoogle Scholar9. Pike RM: The isolation of beta-hemolytic streptococci from throat swabs, Am. J. Hyg. 41: 211, 1945. Google Scholar10. FeldmanHarmon HAW: Comparative effectiveness of direct blood agar and modified Pike's broth for demonstrating beta-hemolytic streptococci of different serological groups, J. Lab. and Clin. Med. 40: 297, 1952. MedlineGoogle Scholar11. Fry RM: Anaerobic methods for the identification of hemolytic streptococci, J. Path. and Bact. 37: 337, 1933. CrossrefGoogle Scholar12. KrumwiedeKuttner EAG: A growth inhibitory substance for the influenza group of organisms in the blood of various animal species, J. Exper. Med. 67: 429, 1938. CrossrefMedlineGoogle Scholar13. QuinnDennyRiley RWFWHD: Natural occurrence of hemolytic streptococci in normal school children, Am. J. Pub. Health 47: 995, 1957. CrossrefGoogle Scholar14. SchaubMazeikaLeeDunnLachainePrice IGIRMTRAWH: Ecologic studies of rheumatic fever and rheumatic heart disease. Procedure for isolating beta-hemolytic streptococci, Am. J. Hyg. 67: 46, 1958. MedlineGoogle Scholar15. Todd EW: Antihemolysin titers in hemolytic streptococcal infections and their significance in rheumatic fever, Brit. J. Exper. Path. 13: 248, 1932. Google Scholar16. MoteJones JRTD: Studies of hemolytic streptococcal antibodies in control groups, rheumatic fever and rheumatoid arthritis, J. Immunol. 41: 35 and 61, 1941. Google Scholar17. RantzRandallRantz LAEHH: Antistreptolysin-O. A study of this antibody in health and in hemolytic streptococcus respiratory disease in man, Am. J. Med. 5: 3, 1948. CrossrefMedlineGoogle Scholar18. RantzDiCaprioRandall LAJME: Antistreptolysin-O and antihyaluronidase titers in health and in various diseases, Am. J. M. Sc. 224: 194, 1952. CrossrefMedlineGoogle Scholar19. McCarty M: Present status of diagnostic tests for rheumatic fever, Ann. Int. Med. 37: 1027, 1952. LinkGoogle Scholar20. LemonHamburger HMM: Missed cases and contact carriers among nasal carriers of beta-hemolytic streptococci, J. Immunol. 54: 189, 1946. MedlineGoogle Scholar21. PackerArnoultSprunt HMBDH: A study of hemolytic streptococcal infections in relation to antistreptolysin-O titer changes in orphanage children, J. Pediat. 48: 545, 1956. CrossrefMedlineGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: GEORGES WERNER, Ph.D.; DAVID CORNFELD, M.D.; JOHN P. HUBBARD, M.D., F.A.C.P.; GEOFFREY RAKE, M.D., F.A.C.P.Affiliations: Philadelphia, Pennsylvania*Received for publication June 13, 1958.From the Department of Preventive Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania.†Deceased.‡This study received support from The American Heart Association, The National Institutes of Health, The Heart Association of Southeastern Pennsylvania, E. R. Squibb and Sons, and Wyeth Laboratories.Requests for reprints should be addressed to John P. Hubbard, M.D., Department of Public Health and Preventive Medicine, University of Pennsylvania School of Medicine, Philadelphia 4, Pennsylvania. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byStreptococcal Pharyngitis in Adults: Can It Be Efficiently and Effectively Managed by Remote Control?Edward L. Kaplan, MDComparison of media and techniques for detection of group A streptococci in throat swab specimensAn Evaluation of a Streptococcal Control ProgramDiagnosis of Bacterial Upper Respiratory Infection in ChildrenA Four-Year Study of the Occurrence of Beta-Hemolytic Streptococci in 64 School ChildrenIII. Prophylaxis of poststreptococcal sequelae and bacterial endocarditisEpidemiologic Studies of Streptococcal Infection in School ChildrenA Study of Group A Hemolytic Streptococcus Carriers Among School Children: Part II. Significance of the FindingsCurrent status of prophylaxis against rheumatic feverA Study of Group A Hemolytic Streptococcus Carriers Among School Children:. 1 December 1958Volume 49, Issue 6Page: 1320-1331KeywordsEpidemiologyHeartLongitudinal studiesMedical servicesPharynxPreventive medicineResearch designResearch laboratoriesStreptococcal infectionsStreptococcus ePublished: 1 December 2008 Issue Published: 1 December 1958 PDF downloadLoading ...