June 1, 1992Genes & DevelopmentOpen Access

Nonsense mutations inhibit splicing of MVM RNA in cis when they interrupt the reading frame of either exon of the final spliced product.

View Full Paper

Ask AI

Bookmark

Population

Murine cells infected or transfected with mutant autonomous parvovirus minute virus of mice (MVM)

Comparison

Introduction of translation termination signals… vs Wild-type MVM

Design

Preclinical

Authors

Discussion

Nonsense mutations may inhibit viral RNA splicing via exon definition in murine cells; hypothesis-generating for broader cis-regulatory mechanisms.

Population

Murine cells infected or transfected with mutant autonomous parvovirus minute virus of mice (MVM)

Intervention

Introduction of translation termination signals (nonsense mutations) into MVM genes

Comparator

Wild-type MVM

Outcome

Accumulated steady-state levels of R1 relative to R2 RNAsurrogate

Nonsense mutations inhibit splicing of MVM RNA in cis by influencing the mechanism by which exons are defined in murine cells.

Naeger et al. (1992) studied this question.

View Full Paper

Ask AI

Bookmark

Nonsense mutations may inhibit viral RNA splicing via exon definition in murine cells; hypothesis-generating for broader cis-regulatory mechanisms.