Population
Cloned tail fragment of Dictyostelium discoideum myosin expressed as a fusion protein
Comparison
Phosphorylation with myosin heavy chain kinase II vs Native myosin
Design
Preclinical
Authors
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Enables recombinant myosin fragments for kinase assays; leaves open relevance to mammalian cardiac myosin regulation.
The study demonstrates that cloned fragments of Dictyostelium myosin heavy chain can be specifically phosphorylated at threonine residues in the C-terminal region, similar to native myosin, enabling improved kinase assays.
Wagle et al. (1988) studied this question.
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