Serum response elements activate and cAMP responsive elements inhibit expression of transcription factor Egr-1 in synovial fibroblasts of rheumatoid arthritis patients

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Population

Synovial fibroblasts, lymphocytes, and macrophages from rheumatoid arthritis (RA) patients and controls

Comparison

Mutation of transcription factor binding sites… vs Controls and unmutated promoter constructs

Design

Preclinical

Authors

Discussion

Should not yet alter RA diagnostics; leaves open Egr-1 as therapeutic target for validation.

Population

Synovial fibroblasts, lymphocytes, and macrophages from rheumatoid arthritis (RA) patients and controls

Intervention

Mutation of transcription factor binding sites within the Egr-1 promoter and testing using reporter gene constructs

Comparator

Controls (for cell types) and unmutated promoter constructs

Outcome

Egr-1 promoter activity and Egr-1-encoding mRNA levelssurrogate

Egr-1 overexpression in RA synovial fibroblasts is driven by serum response elements and may serve as a diagnostic marker.

Aicher et al. (1999) studied this question.

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