What does this research mean for the field?

Hepatic lipid accumulation in response to trans-10,cis-12 conjugated linoleic acid involves both direct effects via the mTOR pathway and indirect, free fatty acid-mediated effects via AMPK and JNK signaling pathways. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To determine whether circulating free fatty acids indirectly contribute to trans-10,cis-12 conjugated linoleic acid (t10,c12 CLA)-induced hepatic lipid accumulation via distinct signaling pathways.

August 23, 2026Nutrition & MetabolismOpen Access

Distinct contributions of mTOR and AMPK/JNK to trans-10,cis-12 conjugated linoleic acid-induced hepatic lipid accumulation in lean and db/db mice

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To determine whether circulating free fatty acids indirectly contribute to trans-10,cis-12 conjugated linoleic acid (t10,c12 CLA)-induced hepatic lipid accumulation via distinct signaling pathways.
Fed lean and obese db/db mice a control diet or a 0.4% w/w t10,c12 CLA diet for 4 weeks to evaluate lipid accumulation and regulatory pathway markers.
Assessed hepatic lipid uptake (CD36, FABP1), synthesis (ACC, FAS), secretion (MTP), and intracellular signaling markers (mTOR, AMPK, PPARα, JNK).
Treated rat H4IIE hepatoma cells with t10,c12 CLA or oleic acid to isolate direct fatty acid effects from indirect free fatty acid-induced signaling in vitro.
Dietary t10,c12 CLA increased hepatic lipid accumulation, FAS expression, and PPARα in lean mice, but did not increase lipid accumulation in db/db mice with established steatosis.
mTOR signaling (S6RP) and FABP1 increased in both lean and db/db mice, whereas MTP, JNK, and AMPK activation were greater in db/db mice.
In hepatoma cells, t10,c12 CLA specifically activated mTOR signaling, whereas oleic acid exposure stimulated AMPK, p-ACC, and cellular stress signaling via JNK.

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Implication

Comparative animal study demonstrates that conjugated linoleic acid drives hepatic fat buildup via mTOR and FFA signaling in mice, indicating distinct direct and indirect metabolic pathways.

Cite This Study

Cordero-Monroy et al. (2026) studied this question.

synapsesocial.com/papers/6a8aadf37677a3411444693c https://doi.org/10.1186/s12986-026-01189-y

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Implication

Comparative animal study demonstrates that conjugated linoleic acid drives hepatic fat buildup via mTOR and FFA signaling in mice, indicating distinct direct and indirect metabolic pathways.

Distinct contributions of mTOR and AMPK/JNK to trans-10,cis-12 conjugated linoleic acid-induced hepatic lipid accumulation in lean and db/db mice

Key Points

Discussion

Implication

Cite This Study

Distinct contributions of mTOR and AMPK/JNK to trans-10,cis-12 conjugated linoleic acid-induced hepatic lipid accumulation in lean and db/db mice

Key Points

Discussion

Implication

Cite This Study

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