Inhibition of renal cortical Rb+ transport by phospholipase C and its relationship to (Na++K+)‐adenosine triphosphatase

Treatment of isolated renal cortical tubules with a low concentration of phospholipase C (one which does not lead to a chemically detectable loss of tubular phospholipid) results in a markedly inhibited Rb+ transport function which is referable to the loss of a phospholipid component of the tubules. This treatment also results in an inhibition of the (Na++K+)‐adenosine triphosphatase enzyme of the tubules. The data are consistent with the concept that it is the loss in (Na++K+)‐adenosine triphosphatase activity which is responsible for phospholipase C‐induced inhibition of Rb+ transport.