What question did this study set out to answer?

To investigate the direct effects of the synthetic glucocorticoid dexamethasone on carbohydrate and lipid metabolism in isolated rat adipose tissue.

November 1, 1967Journal of Endocrinology

The Influence of Dexamethasone on Adipose Tissue Metabolism in Vitro

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RYR. E. YorkeScience Oxford

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Implication

In vitro experiment demonstrates dexamethasone suppresses glucose uptake and glycolysis in rat adipose tissue, indicating glucocorticoid-induced metabolic inhibition counteracted by insulin.

Key Points

To investigate the direct effects of the synthetic glucocorticoid dexamethasone on carbohydrate and lipid metabolism in isolated rat adipose tissue.
Incubated rat epididymal fat pads in vitro with dexamethasone, with metabolic assessments including pre-incubation periods of up to 2 hours.
Measured glucose uptake kinetics, lactate and pyruvate production, tissue metabolite concentrations, and [1-14C]glycine incorporation into protein and nucleic acid fractions.
Tested the reversibility of dexamethasone-induced effects using insulin (500 μ-u./ml).
Dexamethasone decreased the maximal rate of glucose uptake (Vmax) from 0.63 to 0.31 mg/g wet tissue/hr without altering the affinity constant Ku (0.29 mg/ml).
Dexamethasone reduced lactate and pyruvate outputs, the medium lactate:pyruvate ratio, and tissue levels of glycogen, glucose-6-phosphate, and l-glycerol-3-phosphate, without altering adenine nucleotides, citrate, or glycerol output.
Metabolic inhibition emerged after a 2-hour pre-incubation latency period and was rapidly and completely reversed by insulin (500 μ-u./ml).

Cite This Study

R. E. Yorke (1967) studied this question.

synapsesocial.com/papers/6a8ac3a9f8241cb45bfb3614 https://doi.org/10.1677/joe.0.0390329

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Implication

In vitro experiment demonstrates dexamethasone suppresses glucose uptake and glycolysis in rat adipose tissue, indicating glucocorticoid-induced metabolic inhibition counteracted by insulin.

The Influence of Dexamethasone on Adipose Tissue Metabolism in Vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

The Influence of Dexamethasone on Adipose Tissue Metabolism in Vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

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