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December 1, 1989Journal of VirologyOpen Access

A cis-acting mutation in the Sindbis virus junction region which affects subgenomic RNA synthesis

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Population

Chicken embryo fibroblasts or BHK cells transfected with Sindbis virus RNA transcripts

Comparison

Three-base insertion mutation in the junction… vs Wild-type Sindbis virus

Design

Preclinical

Authors

AGArash GrakouiEmory UniversityRLRobin LevisUnited States Food and Drug AdministrationRRRajesh RajuYenepoya University

Discussion

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Implication

Hypothesis-generating for alphavirus promoter mapping; leaves open validation in mammalian models and therapeutic targeting.

Structured PICO

P
Population
Chicken embryo fibroblasts or BHK cells transfected with Sindbis virus RNA transcripts
I
Intervention
Three-base insertion mutation in the junction region (Toto1100CR4.1 mutant)
C
Comparator
Wild-type Sindbis virus
O
Outcome
Level of subgenomic RNA synthesissurrogate

A three-base insertion in the Sindbis virus junction region drastically reduces subgenomic RNA synthesis, demonstrating its role as a cis-acting promoter element recognized by viral trans-acting components.

Cite This Study

Grakoui et al. (1989) studied this question.

synapsesocial.com/papers/6a8acfcd95f0361a9ebe95c0https://doi.org/10.1128/jvi.63.12.5216-5227.1989
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