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September 1, 1990Journal of VirologyOpen Access

Defined mutations in the 5' nontranslated sequence of Sindbis virus RNA

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Key result

Deletions in the first 44 nucleotides of the 5' nontranslated region of Sindbis virus RNA were either lethal or resulted in poorly growing virus, demonstrating this hairpin structure is essential for replication.

Population

Chicken embryo fibroblasts and mosquito C6/36 cells from Aedes albopictus

Comparison

24 deletion mutants and substitution mutants in… vs Parental HRSP virus (wild-type)

Design

Preclinical

Authors

HNHubert G.M. NiestersCalifornia Institute of TechnologyJSJames H. StraussCalifornia Institute of Technology

Discussion

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Implication

Suggests alphavirus antiviral targets; leaves open in vivo efficacy and host-specific translation.

Structured PICO

P
Population
Chicken embryo fibroblasts and mosquito C6/36 cells from Aedes albopictus
I
Intervention
24 deletion mutants and substitution mutants in the 5' nontranslated region of Sindbis virus RNA
C
Comparator
Parental HRSP virus (wild-type)
O
Outcome
Virus replication (assessed by plaque formation and one-step growth curves)surrogate

The 5'-terminal hairpin structure of Sindbis virus RNA is critical for viral replication and interacts differently with host factors in mosquito versus chicken cells.

Limitations

  • SP6 polymerase may introduce changes during RNA transcription, requiring caution in interpretation.
  • Attempts to sequence the RNA from 5NTd(1) were inconclusive.

Cite This Study

Niesters et al. (1990) studied Sindbis virus. Defined mutations in the 5' nontranslated sequence vs. Parental HRSP strain was evaluated on Virus replication rate. Deletions in the first 44 nucleotides of the 5' nontranslated region of Sindbis virus RNA were either lethal or resulted in poorly growing virus, demonstrating this hairpin structure is essential for replication.

synapsesocial.com/papers/6a93aefd57eb2ec944696cb6https://doi.org/10.1128/jvi.64.9.4162-4168.1990
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs1984 · 3,342 citations
  2. 2Effects of 5'-terminal modifications on the biological activity of defective interfering RNAs of Sindbis virus1988 · 24 citations
  3. 3A cis-acting mutation in the Sindbis virus junction region which affects subgenomic RNA synthesis1989 · 63 citations
  4. 4Mutagenesis of the conserved 51-nucleotide region of Sindbis virus1990 · 87 citations
  5. 5Mutagenesis of the 3' nontranslated region of Sindbis virus RNA1990 · 129 citations