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January 1, 1988Journal of VirologyOpen Access

Effects of 5'-terminal modifications on the biological activity of defective interfering RNAs of Sindbis virus

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Population

Defective interfering (DI) genomes of the RNA enveloped virus Sindbis virus

Comparison

5'-terminal modifications vs Naturally occurring DI RNAs or unmodified…

Design

Preclinical

Authors

MTManuel TsiangGilead Sciences (United States)BWB WeissCleveland Clinic FloridaSSSondra SchlesingerWashington University in St. Louis

Discussion

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Implication

Specific 5' deletions dictate Sindbis DI RNA activity; leaves open tertiary structure requirements for alphavirus replication studies.

Structured PICO

P
Population
Defective interfering (DI) genomes of the RNA enveloped virus Sindbis virus
I
Intervention
5'-terminal modifications (deletions and mutations in cDNAs transcribing DI RNAs)
C
Comparator
Naturally occurring DI RNAs or unmodified transcripts
O
Outcome
Biological activity (replication/amplification) of DI RNAs

Tertiary structure or RNA-protein interaction adaptability, rather than common secondary structures, appears crucial for the recognition and biological activity of Sindbis virus defective interfering RNAs.

Cite This Study

Tsiang et al. (1988) studied this question.

synapsesocial.com/papers/6a9458346d20e72ae370776fhttps://doi.org/10.1128/jvi.62.1.47-53.1988
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Establishment and maintenance of persistent infection by Sindbis virus in BHK cells1980 · 66 citations
  2. 2DNA sequencing with chain-terminating inhibitors1977 · 69,520 citations
  3. 3Studies of defective interfering RNAs of Sindbis virus with and without tRNAAsp sequences at their 5' termini1985 · 41 citations
  4. 4RNAs from two independently isolated defective interfering particles of Sindbis virus contain a cellular tRNA sequence at their 5' ends.1983 · 115 citations
  5. 5Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.1985 · 809 citations