What question did this study set out to answer?

To characterize the secondary structure and folding conformation of the immunoglobulin J chain to clarify its role in the synthesis and mucosal transport of polymeric antibodies.

September 1, 1985Proceedings of the National Academy of SciencesOpen Access

Secondary structure of the immunoglobulin J chain.

View Full Paper

Ask AI

Bookmark

To characterize the secondary structure and folding conformation of the immunoglobulin J chain to clarify its role in the synthesis and mucosal transport of polymeric antibodies.
Conducted computer-assisted secondary structure predictions using human and mouse J-chain amino acid sequences.
Performed circular dichroism spectroscopy on isolated J chain across denatured, S-carboxymethylated, S-sulfonated, and air-oxidized states.
Sequence analysis predicted an eight-stranded antiparallel beta-barrel containing approximately 37% beta-sheet conformation with the remainder forming reverse turns and random coil.
Circular dichroism measurements showed denatured and modified J chains contained 25% beta-structure and 75% random coil, which increased to 34% beta-structure after disulfide bond reformation by air oxidation.
Structural comparisons supported a single-domain antiparallel beta-sheet bilayer model over the previously proposed two-domain model.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Biochemical analysis demonstrates an antiparallel beta-barrel conformation in isolated immunoglobulin J chains, suggesting a single-domain folding model for polymeric antibody assembly.

Cite This Study

Zikán et al. (1985) studied this question.

synapsesocial.com/papers/6a8adc300c58d86fb4ca9f2b https://doi.org/10.1073/pnas.82.17.5905

View Full Paper

Ask AI

Bookmark

Implication

Biochemical analysis demonstrates an antiparallel beta-barrel conformation in isolated immunoglobulin J chains, suggesting a single-domain folding model for polymeric antibody assembly.

Secondary structure of the immunoglobulin J chain.

Key Points

Discussion

Implication

Cite This Study

Secondary structure of the immunoglobulin J chain.

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider