January 1, 2010Platelets

GPIbα-selective activation of platelets induces platelet signaling events comparable to GPVI activation events

Q: What is the clinical evidence from this study?

Study design: Other. Intervention: Selective engagement of platelet GPIbalpha by VWF-A1/R543W cells vs. Engagement of GPVI by collagen or collagen-related peptide (CRP). Primary outcome: Platelet aggregation and signaling (tyrosine phosphorylation of Syk).

View Full Paper

Ask AI

Bookmark

Key result

Selective engagement of platelet GPIbalpha by VWF-A1/R543W cells caused rapid, spontaneous platelet aggregation and tyrosine phosphorylation of Syk comparable to GPVI activation by collagen.

Population

Washed platelets and COS-7 cells expressing the VWF-A1 domain containing an R543W mutation

Comparison

Selective engagement of platelet GPIbα by… vs Engagement of GPVI by collagen or…

Design

Preclinical

Authors

EGElizabeth E. Gardiner JAJane F. Arthur YSYang Shen

Discussion

Member takes

Overview

GPIb-IX-V functions as both adhesion and signaling receptor; extends mechanistic models in animals but leaves open human translation.

Structured PICO

Population

Washed platelets and COS-7 cells expressing the VWF-A1 domain containing an R543W mutation

Intervention

Selective engagement of platelet GPIbα by VWF-A1/R543W cells

Comparator

Engagement of GPVI by collagen or collagen-related peptide (CRP)

Outcome

Platelet aggregation and signaling (tyrosine phosphorylation patterns and specific tyrosine phosphorylation of Syk)surrogate

Specific ligation of GPIbα leads to robust platelet activation, defining GPIb-IX-V as both an adhesion and signaling receptor.

Cite This Study

Gardiner et al. (2010) studied this question. Selective engagement of platelet GPIbalpha by VWF-A1/R543W cells vs. Engagement of GPVI by collagen or collagen-related peptide (CRP) was evaluated on Platelet aggregation and signaling (tyrosine phosphorylation of Syk). Selective engagement of platelet GPIbalpha by VWF-A1/R543W cells caused rapid, spontaneous platelet aggregation and tyrosine phosphorylation of Syk comparable to GPVI activation by collagen.

synapsesocial.com/papers/6a8b1814fe910689a2892fa2 https://doi.org/10.3109/09537101003695339

View Full Paper

Ask AI

Bookmark

January 1, 2010Platelets

GPIbα-selective activation of platelets induces platelet signaling events comparable to GPVI activation events

View Full Paper

Ask AI

Bookmark

Key result

Selective engagement of platelet GPIbalpha by VWF-A1/R543W cells caused rapid, spontaneous platelet aggregation and tyrosine phosphorylation of Syk comparable to GPVI activation by collagen.

Population

Washed platelets and COS-7 cells expressing the VWF-A1 domain containing an R543W mutation

Comparison

Selective engagement of platelet GPIbα by… vs Engagement of GPVI by collagen or…

Design

Preclinical

Authors

EGElizabeth E. Gardiner JAJane F. Arthur YSYang Shen

Discussion

Member takes

Overview

GPIb-IX-V functions as both adhesion and signaling receptor; extends mechanistic models in animals but leaves open human translation.

Structured PICO

Population

Washed platelets and COS-7 cells expressing the VWF-A1 domain containing an R543W mutation

Intervention

Selective engagement of platelet GPIbα by VWF-A1/R543W cells

Comparator

Engagement of GPVI by collagen or collagen-related peptide (CRP)

Outcome

Platelet aggregation and signaling (tyrosine phosphorylation patterns and specific tyrosine phosphorylation of Syk)surrogate

Specific ligation of GPIbα leads to robust platelet activation, defining GPIb-IX-V as both an adhesion and signaling receptor.

Cite This Study

synapsesocial.com/papers/6a8b1814fe910689a2892fa2 https://doi.org/10.3109/09537101003695339

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark