Time-irreversible Subconductance Gating Associated with Ba2+ Block of Large Conductance Ca2+-activated K+ Channels

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Population

Patches of membrane excised from cultures of rat skeletal muscle

Comparison

Ba2+ block under specific conditions vs Normal gating in the absence of Ba2+, and…

Design

Preclinical

Authors

Discussion

Hypothesis-generating for Ba2+ effects on BK channel gating; leaves open relevance to human physiology or therapy.

Population

Patches of membrane excised from cultures of rat skeletal muscle

Intervention

Ba2+ block (10 microM Ba2+i or 10 mM Ba2+o) under specific conditions (150 mM N-methyl-D-glucamine+o, 500 mM K+i, +30 mV, 100 microM Ca2+i)

Comparator

Normal gating in the absence of Ba2+, and external solutions with added K+, Rb+, or Na+

Outcome

Characteristics of Ba2+ blocks including duration, termination with subconductance level (preopening), and fractional conductancesurrogate

The study demonstrates that Ba2+ block of large conductance Ca2+-activated K+ channels induces time-irreversible subconductance gating (preopenings).

Bello et al. (1998) studied this question.

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