What question did this study set out to answer?

To determine the anion dependence and cellular transport mechanisms governing acidification in the rabbit medullary collecting duct.

May 1, 1983Journal of Clinical InvestigationOpen Access

Anion dependence of rabbit medullary collecting duct acidification.

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Authors

DSDennis K. StoneThe University of Texas Southwestern Medical Center DSDonald W. SeldinThe University of Texas Southwestern Medical Center JKJuha P. KokkoNational Defence University

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Implication

Ex vivo perfusion study demonstrates chloride-dependent bicarbonate reabsorption in rabbit medullary collecting ducts, indicating basolateral anion exchange couples electrogenic proton secretion.

Key Points

To determine the anion dependence and cellular transport mechanisms governing acidification in the rabbit medullary collecting duct.
Perfused isolated rabbit medullary collecting ducts ex vivo to monitor net rates of tubular bicarbonate reabsorption and chloride reabsorption.
Replaced luminal perfusate and peritubular bath chloride with gluconate, and administered the anion exchange inhibitor SITS to either luminal or basolateral surfaces.
Total replacement of luminal chloride with gluconate increased bicarbonate reabsorption from 10.7 ± 1.0 to 15.01 ± 1.0 pmol · mm⁻¹ · min⁻¹, whereas bath chloride removal abolished acidification.
Basolateral addition of SITS reduced bicarbonate reabsorption from 10.1 ± 1.2 to 2.3 ± 0.3 pmol · mm⁻¹ · min⁻¹, whereas luminal SITS had no inhibitory effect.
Chloride reabsorption rate (9.75 ± 1.18 pmol · mm⁻¹ · min⁻¹) demonstrated colinearity with bicarbonate reabsorption rate (9.18 ± 0.9 pmol · mm⁻¹ · min⁻¹).

Cite This Study

Stone et al. (1983) studied this question.

synapsesocial.com/papers/6a8b26d20ca85c7e33dee1dc https://doi.org/10.1172/jci110905

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Implication

Ex vivo perfusion study demonstrates chloride-dependent bicarbonate reabsorption in rabbit medullary collecting ducts, indicating basolateral anion exchange couples electrogenic proton secretion.

Anion dependence of rabbit medullary collecting duct acidification.

Discussion

Member takes

Implication

Key Points

Cite This Study

Anion dependence of rabbit medullary collecting duct acidification.

Discussion

Member takes

Implication

Key Points

Cite This Study

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