Key result
Responses of rat aorta to lower concentrations of ET-1 involve only NSCC-2, whereas higher concentrations involve NSCC-1, NSCC-2, and SOCC, contributing 10%, 55%, and 35% to total Ca2+ entry.
Population
Rat thoracic aortic smooth muscle cells (A7r5 cells) and rat thoracic aorta
Comparison
Endothelin-1 stimulation with Ca2+ channel… vs Control (ET-1 stimulation without blockers)
Design
Preclinical
Authors
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Findings in rat aorta warrant caution before clinical translation; extends mechanistic insight into concentration-dependent Ca2+ entry in rodent models.
Endothelin-1 activates distinct voltage-independent Ca2+ entry channels (NSCC-1, NSCC-2, and SOCC) in rat aortic smooth muscle cells in a concentration-dependent manner.
Miwa et al. (1999) studied this question. Endothelin-1 (ET-1) and Ca2+ channel blockers (SK&F 96365, LOE 908) vs. controls was evaluated on Ca2+ entry channels involved in ET-1-induced contractions and increases in intracellular free Ca2+ concentration. Responses of rat aorta to lower concentrations of ET-1 involve only NSCC-2, whereas higher concentrations involve NSCC-1, NSCC-2, and SOCC, contributing 10%, 55%, and 35% to total Ca2+ entry.
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