The liver phosphoenolpyruvate carboxykinase activity and blood glucose and ketone concentrations of 18 dairy cows were determined at 6 weeks prepartum, during the first week postpartum, and during the sixth week postpartum. Six of the cows were assigned to each of 3 treatments: a) hay ad libitum up to parturition supplemented with restricted con-, centrate (15% protein) during lactation; b) restricted hay plus concentrate (15% protein) to appetite immediately before parturition and during lactation; and c) same as b with a 24% protein concentrate. The only significant change in liver phosphoenolpyruvate carboxykinasc activity was a reduction in the activity of the cows on Treatment A during the first week postpartum. At the same time blood glucose was depressed and blood ketones elevated. The liver enzyme activity was positively correlated with digestible dry matter intake, crude protein intake, milk production and body weight:
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Butler et al. (1970) studied this question.
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