November 13, 2014PLoS ONEOpen Access

Ca2+-Induced PRE-NMR Changes in the Troponin Complex Reveal the Possessive Nature of the Cardiac Isoform for Its Regulatory Switch

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Population

Cardiac Troponin complex (cTnC-cTnI binary constructs)

Comparison

Paramagnetic relaxation enhancement-NMR with… vs Ca2+-bound vs Ca2+-free states

Design

Preclinical

Authors

NCNicole M. CordinaMacquarie University CLChu Kong LiewVictor Chang Cardiac Research Institute PPPhani R. PotluriMacquarie University

Discussion

Member takes

Implication

Highlights troponin flexibility in Ca-free state; leaves open role in human cardiac kinetics and therapeutic targeting.

Structured PICO

Population

Cardiac Troponin complex (cTnC-cTnI binary constructs)

Intervention

Paramagnetic relaxation enhancement (PRE)-NMR with site-directed spin-labeling

Comparator

Ca2+-bound vs Ca2+-free states

Outcome

Conformational changes and movement of key regions of the troponin I (cTnI) subunit upon the release of Ca2+ from Troponin C (cTnC)surrogate

PRE-NMR structural analysis reveals that the cardiac troponin I regulatory switch region remains highly flexible and in close proximity to troponin C in the calcium-free state, which may facilitate rapid cardiac muscle activation.

Cite This Study

Cordina et al. (2014) studied this question.

synapsesocial.com/papers/6a8bb0f4f71b672e3df48e45 https://doi.org/10.1371/journal.pone.0112976

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Ca2+-Induced PRE-NMR Changes in the Troponin Complex Reveal the Possessive Nature of the Cardiac Isoform for Its Regulatory Switch

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Ca2+-Induced PRE-NMR Changes in the Troponin Complex Reveal the Possessive Nature of the Cardiac Isoform for Its Regulatory Switch

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider