May 1, 1990CellOpen Access

Destruction of U2, U4, or U6 small nuclear RNA blocks Trans splicing in trypanosome cells

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Population

Permeable cells of Trypanosoma brucei

Design

Preclinical

Authors

Discussion

U snRNAs required for early trans splicing in trypanosomes; leaves open extension to other eukaryotes or therapeutic targets.

Population

Permeable cells of Trypanosoma brucei

Intervention

Degradation of U2, U4, or U6 snRNA by site-directed cleavage with complementary deoxyoligonucleotides and RNAase H

Outcome

Trans splicing of the spliced leader (SL) RNA and newly synthesized alpha-tubulin pre-mRNAssurrogate

The study provides direct evidence for the participation of U2 and U4/U6 snRNPs in the early steps of trans splicing in trypanosomes.

Tschudi et al. (1990) studied this question.

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