Key result
In vivo UV cross-linking in Trypanosoma brucei revealed RNA-RNA interactions between U2 and U6 RNAs, and U4 and U6 RNAs, consistent with base-pairing between the 5' domain of U2 and 3' end of U6.
Population
Trypanosoma brucei (in vivo model)
Design
Preclinical
Authors
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SL-U snRNA cross-links in T. brucei support trans-splicing models; leaves open exact sites for functional validation.
The study reveals in vivo cognate RNA-RNA interactions in RNA homologs participating in trans-splicing in trypanosomes.
Watkins et al. (1991) studied Trypanosoma brucei RNA trans-splicing. In vivo UV cross-linking was evaluated on RNA-RNA interactions between SL RNA and U snRNAs. In vivo UV cross-linking in Trypanosoma brucei revealed RNA-RNA interactions between U2 and U6 RNAs, and U4 and U6 RNAs, consistent with base-pairing between the 5' domain of U2 and 3' end of U6.
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