What question did this study set out to answer?

To determine the dephosphorylation kinetics of vimentin phosphorylation sites Ser38 and Ser82 and elucidate the structural basis for prolonged Ser82 phosphorylation in astrocytes.

March 28, 2006Genes to CellsOpen Access

Vimentin‐Ser82 as a memory phosphorylation site in astrocytes

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To determine the dephosphorylation kinetics of vimentin phosphorylation sites Ser38 and Ser82 and elucidate the structural basis for prolonged Ser82 phosphorylation in astrocytes.
Induced CaMKII-mediated vimentin phosphorylation in astrocytes using PGF(2alpha) or ionomycin.
Assayed in vitro dephosphorylation kinetics of phospho-Ser38 and phospho-Ser82 using purified PP1 catalytic subunit (PP1c) and mapped PP1c binding to the vimentin VxF motif.
Examined in vivo PP1calpha-vimentin association and structural filament disassembly following repeated ionomycin administration in astrocytes.
Phospho-Ser82 dephosphorylated significantly slower than phospho-Ser38 and was insensitive to in vitro dephosphorylation by purified PP1c.
PP1c bound directly to the adjacent vimentin VxF motif (Val83-Asp84-Phe85), sterically obstructing its active site from accessing phospho-Ser82 and prolonging phosphorylation.
Repetitive ionomycin exposure at short intervals induced sustained Ser82 phosphorylation, triggering pronounced disassembly of astrocytic intermediate filaments.

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Implication

Experimental study demonstrates prolonged Ser82 phosphorylation of vimentin in astrocytes, indicating a memory phosphorylation mechanism regulating intermediate filament disassembly.

Cite This Study

Oguri et al. (2006) studied this question.

synapsesocial.com/papers/6a8bcc9c6d4d918f27dec2e9 https://doi.org/10.1111/j.1365-2443.2006.00961.x

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Implication

Experimental study demonstrates prolonged Ser82 phosphorylation of vimentin in astrocytes, indicating a memory phosphorylation mechanism regulating intermediate filament disassembly.

Vimentin‐Ser82 as a memory phosphorylation site in astrocytes

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Discussion

Implication

Cite This Study

Vimentin‐Ser82 as a memory phosphorylation site in astrocytes

Key Points

Discussion

Implication

Cite This Study

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