What question did this study set out to answer?

Develop and assess a solid-phase enzyme immunoassay using alkaline phosphatase conjugated to anti-immunoglobulin for the sensitive detection of influenza A antibodies.

December 1, 1976Journal of Clinical PathologyOpen Access

Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin.

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Authors

PLPauli LeinikkiInstitute for Molecular Medicine Finland SPS. Passila

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Implication

Assay validation study reveals high sensitivity of solid-phase enzyme immunoassay for influenza A antibodies in vaccinated individuals, indicating faster clinical serological diagnosis.

Key Points

Develop and assess a solid-phase enzyme immunoassay using alkaline phosphatase conjugated to anti-immunoglobulin for the sensitive detection of influenza A antibodies.
Sensitized custom microcuvettes with influenza A antigen to capture serum antibodies from vaccine recipients.
Quantified bound antibodies using an anti-IgG alkaline phosphatase conjugate in a semiautomated photometer coupled to a programmable calculator.
Evaluated sensitivity and analytical reproducibility against standard hemagglutination inhibition (HI) and complement fixation (CF) assays.
Achieved 200-fold higher analytical sensitivity compared to conventional HI and CF methods.
Demonstrated low interassay variability, rendering twofold differences in antibody concentration statistically significant.
Identified diagnostic rises in antibody titers within three to six days post-vaccination, facilitating accelerated clinical serodiagnosis.

Cite This Study

Leinikki et al. (1976) studied this question.

synapsesocial.com/papers/6a8be80ca42d46e0ffcad533 https://doi.org/10.1136/jcp.29.12.1116

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Implication

Assay validation study reveals high sensitivity of solid-phase enzyme immunoassay for influenza A antibodies in vaccinated individuals, indicating faster clinical serological diagnosis.

Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin.

Discussion

Member takes

Implication

Key Points

Cite This Study

Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin.

Discussion

Member takes

Implication

Key Points

Cite This Study

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