Why the study?
Does the use of monoclonal antibodies improve the sensitivity of radioimmunoassay and enzyme-linked immunosorbent assay for detecting hepatitis A virus and antibodies compared to human polyclonal serum?
Does the use of monoclonal antibodies improve the sensitivity of radioimmunoassay and enzyme-linked immunosorbent assay for detecting hepatitis A virus and antibodies compared to human polyclonal serum?
Monoclonal antibody-based assays offer higher sensitivity for detecting hepatitis A virus and its antibodies compared to polyclonal serum-based assays.
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Monoclonal antibody assays may enhance HAV detection in labs; leaves open prospective validation before routine clinical adoption.
Monoclonal antibodies (K3-2F2 and K3-4C8) raised against hepatitis A virus were used to develop a solid-phase radioimmunoassay and enzyme-linked immunosorbent assay for the detection of hepatitis A virus and antibody. Assays with this pair of monoclonal antibodies were compared in parallel with similarly constructed solid-phase radioimmunoassays and enzyme-linked immunosorbent assays in which human polyclonal serum was used. The monoclonal antibody assay proved to be more sensitive for the detection of hepatitis A virus from fecal specimens as well as for anti-hepatitis A virus immunoglobulin G (IgG) and IgM in sera.
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Coulepis et al. (1985) studied this question.
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