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May 1, 1977Journal of Clinical MicrobiologyOpen Access

Serodiagnosis of viral hepatitis A: detection of acute-phase immunoglobulin M anti-hepatitis A virus by radioimmunoassay

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Why the study?

Does a modified micro solid-phase radioimmunoassay accurately detect acute-phase IgM anti-hepatitis A virus to diagnose viral hepatitis A?

Population

Coded human and chimpanzee serum specimens, including early-acute-phase serum specimens from human cases of…

Design

Preclinical, Coded human and chimpanzee serum specimens

Authors

DBDaniel W. BradleyEcological Society of AmericaJMJames E. MaynardMathematical Institute of the Slovak Academy of SciencesSHS. HindmanArizona Department of Health Services

Discussion

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Overview

Promising for minimal-sample acute hepatitis A diagnosis; leaves open human validation from animal data.

Structured PICO

Does a modified micro solid-phase radioimmunoassay accurately detect acute-phase IgM anti-hepatitis A virus to diagnose viral hepatitis A?

P
Population
Coded human and chimpanzee serum specimens, including early-acute-phase serum specimens from human cases of hepatitis A and acute-phase sera from experimentally infected chimpanzees
I
Intervention
Modified micro solid-phase radioimmunoassay (RIA) for antibody to hepatitis A virus (anti-HAV), including an adapted RIA-IgM blocking procedure
O
Outcome
Detection of acute-phase immunoglobulin M (IgM) anti-hepatitis A virus (specificity and sensitivity of the RIA procedure)surrogate

A modified radioimmunoassay can effectively detect acute-phase IgM anti-HAV using less than 1 microliter of serum, enabling rapid serodiagnosis of acute viral hepatitis A.

Cite This Study

Bradley et al. (1977) studied this question.

synapsesocial.com/papers/6a842eb074ee596ff2c52077https://doi.org/10.1128/jcm.5.5.521-530.1977
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Purification of hepatitis A virus from chimpanzee stools1977 · 13 citations
  2. 2Hepatitis A Antigen Particles in Liver, Bile, and Stool of Chimpanzees1976 · 101 citations
  3. 3Comparison of serological tests for antibody to hepatitis A antigen, using coded specimens from individuals infected with the MS-1 strain of hepatitis A virus1976 · 11 citations
  4. 4Purification of hepatitis A antigen from feces and detection of antigen and antibody by immune adherence hemagglutination1976 · 109 citations
  5. 5Hepatitis A: Detection by Immune Electron Microscopy of a Viruslike Antigen Associated with Acute Illness1973 · 867 citations