July 1, 1985AJP Cell Physiology

Very small fat cell populations determined by a modified osmium tetroxide-urea method

View Full Paper

Ask AI

Bookmark

Population

Fischer 344 epididymal and Sprague-Dawley retroperitoneal adipose depots

Comparison

Modified osmium tetroxide-8M urea method vs Collagenase method

Design

Preclinical

Authors

FDFrederick D. DeMartinisLos Alamos National Laboratory

Discussion

Member takes

Implication

Facilitates VSFC isolation in animal models; leaves open translation to human adipose research.

Structured PICO

Population

Fischer 344 epididymal and Sprague-Dawley retroperitoneal adipose depots

Intervention

Modified osmium tetroxide-8M urea method (addition of a 95% ethanol step, centrifugation, and final resuspension in 55% glycerol in 0.01% Triton-saline)

Comparator

Collagenase method

Outcome

Determination of very small fat cell (VSFC) populations (adipocyte diameters measured by microscopy)surrogate

A modified osmium tetroxide-urea method is superior to the collagenase method for identifying and studying very small fat cell populations in adipose tissue.

Cite This Study

F D DeMartinis (1985) studied this question.

synapsesocial.com/papers/6a8bea4a52e1a91077e984ec https://doi.org/10.1152/ajpcell.1985.249.1.c89

View Full Paper

Ask AI

Bookmark

Very small fat cell populations determined by a modified osmium tetroxide-urea method

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Very small fat cell populations determined by a modified osmium tetroxide-urea method

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider