What question did this study set out to answer?

To evaluate whether a rapid cell-sizing technique using fixed tissue sections reliably estimates human fat cell diameters compared to sizing in cell suspensions.

March 1, 1976Journal of Lipid ResearchOpen Access

Human fat cell sizing—a quick, simple method

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Population

37 samples of human adipose tissue, obtained by open or needle biopsy

Comparison

Method II (sizing cells from fixed sections) vs Method I (sizing cells in cell suspensions)

Authors

MAMargaret AshwellAshwell Museum PPPauline PriestMiddlesex University MBM Bondoux

Discussion

Member takes

Implication

Simplifies adipocyte sizing from fixed sections; leaves open larger validation before clinical or research adoption.

Key Points

To evaluate whether a rapid cell-sizing technique using fixed tissue sections reliably estimates human fat cell diameters compared to sizing in cell suspensions.
Analyzed 37 human adipose tissue specimens collected through open or needle biopsies.
Measured mean cell diameters using two distinct approaches: sizing cells in liquid suspensions (Method I) and sizing cells in fixed histological sections (Method II).
Measurements obtained from fixed sections correlated strongly with cell suspension measurements (r = 0.93, P < 0.001).
Cell diameter values derived from fixed sections were slightly lower than suspension values, requiring a mathematical correction factor for direct equivalence.

Structured PICO

Population

37 samples of human adipose tissue, obtained by open or needle biopsy

Intervention

Method II (sizing cells from fixed sections)

Comparator

Method I (sizing cells in cell suspensions)

Outcome

Mean cell diameter agreement between the two methodssurrogate

A quick, simple method of sizing human fat cells from fixed sections shows good agreement with sizing from cell suspensions.

Cite This Study

Ashwell et al. (1976) studied this question.

synapsesocial.com/papers/6a952d0d95cf49f4209e748e https://doi.org/10.1016/s0022-2275(20)37006-1

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Bookmark

Human fat cell sizing—a quick, simple method

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Human fat cell sizing—a quick, simple method

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

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