In vitro study demonstrates antioxidant activity in Kaempferia galanga rhizome extract, indicating freeze-drying preserves ethyl p-methoxycinnamate for medicinal use.
Key Points
To evaluate the in vitro antioxidant capacity and phytochemical composition of freeze-dried ethanolic extract from Kaempferia galanga rhizomes.
Harvested K. galanga rhizomes from Meghalaya, India, and generated an ethanolic extract via lyophilization (freeze-drying).
Measured antioxidant capacity using DPPH and ABTS radical scavenging assays along with total polyphenol and flavonoid quantification.
Profiled phytocompounds using high-performance thin-layer chromatography (HPTLC) and gas chromatography-mass spectroscopy (GCMS).
Demonstrated antioxidant IC50 values of 1.824 mg/mL for DPPH and 0.307 mg/mL for ABTS assays, with a total polyphenol content of 23.55 ± 0.5 mg GAE/g and total flavonoid content of 100 ± 1.414 mg RE/g dry weight.
HPTLC achieved optimal band separation using a 2-butanol/1-propanol/water mobile phase at a 3:1:1 ratio.
GCMS analysis identified ethyl p-methoxycinnamate as the dominant bioactive constituent, representing 94.87% of the total area composition.