What does this research mean for the field?

Nifuroxazide induces significant genetic and biochemical toxicity in non-target plants like Allium cepa by disrupting DNA integrity, inducing oxidative stress, and interacting with critical cellular proteins and enzymes. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To evaluate the genetic and biochemical toxicities of the antibiotic nifuroxazide in the non-target model plant Allium cepa L. through in vivo assays and molecular docking.

August 24, 2026Scientific ReportsOpen Access

Multi-endpoint toxicological assessment of nifuroxazide through experimental and molecular docking analyses

View Full Paper

Ask AI

Bookmark

Authors

FPFurkan Pala KÇKültiğin Çavuşoğlu EYEmine Yalçın

Discussion

Member takes

Overview

Experimental and in silico analysis reveals nifuroxazide induces genotoxicity and oxidative stress in Allium cepa, indicating ecotoxicological risks of environmental antibiotic contamination.

Key Points

To evaluate the genetic and biochemical toxicities of the antibiotic nifuroxazide in the non-target model plant Allium cepa L. through in vivo assays and molecular docking.
Randomly divided A. cepa bulbs into an untreated control germinated in tap water and three treatment groups exposed to nifuroxazide at concentrations of 0.0179, 0.0357, and 0.0714 mg/L throughout germination.
Evaluated root and leaf tissues for mitotic index, micronucleus frequency, chromosomal abnormalities, DNA damage via Comet assay, malondialdehyde levels, superoxide dismutase and catalase activities, and chlorophyll a/b content.
Conducted molecular docking to assess the binding interactions of nifuroxazide with DNA, tubulin proteins, topoisomerase enzymes, and enzymes involved in chlorophyll biosynthesis.
Nifuroxazide produced significant dose-dependent toxic effects (p < 0.05), with the 0.0714 mg/L concentration causing an approximately 22% reduction in mitotic index, a 55.59% decrease in Head DNA (%), a 1.9-fold decrease in chlorophyll a, and a 3.3-fold decrease in chlorophyll b relative to control.
Exposure at 0.0714 mg/L significantly increased oxidative stress markers, elevating malondialdehyde by 2.6-fold, superoxide dismutase by 1.6-fold, and catalase by 2.0-fold, alongside inducing structural chromosomal abnormalities such as vagrant chromosomes, sticky chromosomes, fragments, and bridges.
Molecular docking verified favorable binding interactions with DNA, tubulins, topoisomerases, and chlorophyll-synthesis enzymes, providing mechanistic support for the observed genetic and biochemical alterations.

Cite This Study

Pala et al. (2026) studied this question.

synapsesocial.com/papers/6a8c005bbca056c88e6df0b2 https://doi.org/10.1038/s41598-026-67731-3

View Full Paper

Ask AI

Bookmark

August 24, 2026Scientific ReportsOpen Access

Multi-endpoint toxicological assessment of nifuroxazide through experimental and molecular docking analyses

View Full Paper

Ask AI

Bookmark

Authors

FPFurkan Pala KÇKültiğin Çavuşoğlu EYEmine Yalçın

Discussion

Member takes

Overview

Experimental and in silico analysis reveals nifuroxazide induces genotoxicity and oxidative stress in Allium cepa, indicating ecotoxicological risks of environmental antibiotic contamination.

Key Points

To evaluate the genetic and biochemical toxicities of the antibiotic nifuroxazide in the non-target model plant Allium cepa L. through in vivo assays and molecular docking.
Randomly divided A. cepa bulbs into an untreated control germinated in tap water and three treatment groups exposed to nifuroxazide at concentrations of 0.0179, 0.0357, and 0.0714 mg/L throughout germination.
Evaluated root and leaf tissues for mitotic index, micronucleus frequency, chromosomal abnormalities, DNA damage via Comet assay, malondialdehyde levels, superoxide dismutase and catalase activities, and chlorophyll a/b content.
Conducted molecular docking to assess the binding interactions of nifuroxazide with DNA, tubulin proteins, topoisomerase enzymes, and enzymes involved in chlorophyll biosynthesis.
Nifuroxazide produced significant dose-dependent toxic effects (p < 0.05), with the 0.0714 mg/L concentration causing an approximately 22% reduction in mitotic index, a 55.59% decrease in Head DNA (%), a 1.9-fold decrease in chlorophyll a, and a 3.3-fold decrease in chlorophyll b relative to control.
Exposure at 0.0714 mg/L significantly increased oxidative stress markers, elevating malondialdehyde by 2.6-fold, superoxide dismutase by 1.6-fold, and catalase by 2.0-fold, alongside inducing structural chromosomal abnormalities such as vagrant chromosomes, sticky chromosomes, fragments, and bridges.
Molecular docking verified favorable binding interactions with DNA, tubulins, topoisomerases, and chlorophyll-synthesis enzymes, providing mechanistic support for the observed genetic and biochemical alterations.

Cite This Study

Pala et al. (2026) studied this question.

synapsesocial.com/papers/6a8c005bbca056c88e6df0b2 https://doi.org/10.1038/s41598-026-67731-3

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark