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Sol-gel derived nanostructured cerium oxide (CeO2) film deposited on gold (Au) electrode has been utilized for physisorption of glucose oxidase (GOx). X-ray diffraction, atomic force microscopy, UV-visible spectroscopy, and electrochemical techniques have been used to characterize sol-gel derived CeO2∕Au electrode and GOx∕CeO2∕Au bioelectrode. The response characteristics of the glucose bioelectrode (GOx∕CeO2∕Au) indicate linearity, detection limit and shelf-life as 50–400mg∕dL, 12.0μM, and 12weeks, respectively. The value of apparent Michaelis–Menten constant (Km) of GOx∕CeO2∕Au bioelectrode has been found to be 13.55μM.
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Ansari et al. (2008) studied this question.
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