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September 1, 1993Journal of VirologyOpen Access

Functional analysis of human foamy virus accessory reading frames

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Population

In vitro cell lines (BHK-21, U-251 MG, HEL) and infectious molecular clones of human foamy virus (HFV)

Comparison

Single and combinatory bel gene mutants of an… vs Wild-type HFV or parental construct (pHSRV)

Design

Preclinical

Authors

GBGerald BaunachBMBernd MaurerHHHeidi Hahn

Discussion

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Overview

In human foamy virus, bel-1 is the only accessory protein necessary for viral replication in vitro, acting solely as a transactivator of transcription without evidence of a posttranscriptional regulatory protein.

Structured PICO

P
Population
In vitro cell lines (BHK-21, U-251 MG, HEL) and infectious molecular clones of human foamy virus (HFV)
I
Intervention
Single and combinatory bel gene mutants of an infectious molecular clone of HFV
C
Comparator
Wild-type HFV or parental construct (pHSRV)
O
Outcome
Viral replication competence in vitro (measured by reverse transcriptase activity, virus titration, and cytopathic effect)surrogate

In human foamy virus, bel-1 is the only accessory protein necessary for viral replication in vitro, acting solely as a transactivator of transcription without evidence of a posttranscriptional regulatory protein.

Limitations

  • Failure to demonstrate postulated beo and bel-2 proteins might be due to a lack in sensitivity of the Western blot and immunofluorescence compared with PCR
  • Proteins may be expressed only in certain narrow phases of the viral replication cycle which were missed

Cite This Study

Baunach et al. (1993) studied this question.

synapsesocial.com/papers/6a8c1006510c7253b5dedda9https://doi.org/10.1128/jvi.67.9.5411-5418.1993
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