Whey Utilization

WHEY UTILIZATION.III could be scaled-up to apply to larger operations.Fifteen L of yeast-whey medium in a 40 L propagator were aerated at 30 L per min under conditions that assured an adequate supply of dissolved oxygen (Wasserman and Hampson, 1960).The oxygen consumption by the yeast was determined with the Beckman Oxygen Analyzer.To permit direct comparison with the values obtained in the smaller propagator, the oxygen data were divided by 30 (the ratio between the medium vol- umes of the two propagators).Figure 3 shows the simi- larity of the oxygen consumption patterns between the two propagations.The peak oxygen demand of S. fragilis growing in a whey medium, approximately 110 ml 02 per L medium per min, is equivalent to 5 mM 02 per L per min.This compares very well with the data of Maxon and Johnson describing a peak demand of 4.8 to 5.7 mM 02 per L per min for Saccharomyces cereviseae growing in a rich glucose medium in a continuous propagation.SUMMARY The oxygen demand pattern of Saccharomyces fragilis growing in a whey medium is described.Peak oxygen demand is approximately 110 ml 02 per L medium per min (300 mm per L per hr).