Key result
The duck plague virus gC protein was successfully expressed in a prokaryotic system, and was found to be a late-stage viral protein that localizes predominantly in the cytoplasm of infected cells.
The successful cloning, expression, and characterization of the duck plague virus gC protein provides a foundation for further functional analysis of this viral envelope gene.
Enables functional studies of duck plague virus gC; leaves open its roles in pathogenesis or vaccine targets.
BACKGROUND: Viral envelope proteins have been proposed to play significant roles in the process of viral infection. RESULTS: In this study, an envelope protein gene, gC (NCBI GenBank accession no. EU076811), was expressed and characterized from duck plague virus (DPV), a member of the family herpesviridae. The gene encodes a protein of 432 amino acids with a predicted molecular mass of 45 kDa. Sequence comparisons, multiple alignments and phylogenetic analysis showed that DPV gC has several features common to other identified herpesvirus gC, and was genetically close to the gallid herpervirus.Antibodies raised in rabbits against the pET32a-gC recombinant protein expressed in Escherichia coli BL21 (DE3) recognized a 45-KDa DPV-specific protein from infected duck embryo fibroblast (DEF) cells. Transcriptional and expression analysis, using real-time fluorescent quantitative PCR (FQ-PCR) and Western blot detection, revealed that the transcripts encoding DPV gC and the protein itself appeared late during infection of DEF cells. Immunofluorescence localization further demonstrated that the gC protein exhibited substantial cytoplasm fluorescence in DPV-infected DEF cells. CONCLUSIONS: In this work, the DPV gC protein was successfully expressed in a prokaryotic expression system, and we presented the basic properties of the DPV gC product for the first time. These properties of the gC protein provided a prerequisite for further functional analysis of this gene.
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Lian et al. (2010) studied Duck plague virus (DPV) infection. Cloning and expression of DPV gC gene vs. Mock-infected cells was evaluated on Identification, expression, and characterization of DPV gC gene and protein. The duck plague virus gC protein was successfully expressed in a prokaryotic system, and was found to be a late-stage viral protein that localizes predominantly in the cytoplasm of infected cells.
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