Plasma-treated alginate hydrogels modulate porcine leukocyte activity in vitro

Introduction: . Cold atmospheric plasma (CAP) generates reactive oxygen and nitrogen species (RONS), which can modulate immune cell behavior and may promote tissue repair. Results: Our findings demonstrate that PTH induce a controlled increase in nitrite and superoxide production in both neutrophil and monocyte cultures, particularly at higher treatment levels, without evidence of lytic cell death or significant loss of viability. PTH application resulted in a modest non-significant decrease in neutrophil metabolic activity without promoting degranulation, while monocyte metabolism remained unaffected. These results suggest that therapeutic doses of CAP elicit moderate oxidative stress capable of activating leukocytes while preserving their functional stability. While their potential to enhance wound healing or tissue repair remains speculative, our findings warrant further investigation for applications in both human and veterinary medicine.