The study characterizes ATP-G-actin aggregates formed at low KCl concentrations and links their formation to the rate of viscosity increase at higher KCl concentrations.
Refines in vitro models of actin assembly; extends biochemical characterization but leaves open cardiac cytoskeletal relevance.
The ATP-G-actin aggregates formed by incubation of ATP-G-actin in 7.5 mM-KCl were characterized by electron-microscopical observation, by high-pressure liquid chromatography and by the study of the 1,N6-etheno-ATP-ATP exchange reaction between the free and the actin-bound nucleotide. In 30 mM-KCl the initial rate of the reduced-viscosity increase is found to be directly related to the amount of the aggregates formed in the course of the preincubation in 7.5 mM-KCl.
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Grazi et al. (1984) studied this question.
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