What question did this study set out to answer?

To determine whether annexin II, in monomeric or heterotetrameric form, functions as a membrane receptor responsible for mediating rapid, non-genomic signaling of 1α,25(OH)2-vitamin D3.

February 5, 2004Journal of Cellular Biochemistry

Evidence that annexin II Is not a putative membrane receptor for 1α,25(OH)2‐vitamin D3

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Authors

MMMathew T. MizwickiUniversity of California, Riverside JBJune E. BishopUniversity of California, Riverside COChristopher J. OliveraUniversity of California, Riverside

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Implication

In vitro binding assays demonstrate lack of specific 1,25-vitamin D3 binding to annexin II, indicating annexin II functions as a downstream substrate rather than a direct membrane receptor.

Key Points

To determine whether annexin II, in monomeric or heterotetrameric form, functions as a membrane receptor responsible for mediating rapid, non-genomic signaling of 1α,25(OH)2-vitamin D3.
Assessed in vitro binding affinity of [3H]1,25(OH)2-D3 for annexin II monomer and heterotetramer [AII(2)/p11(2)] across 30 experiments with and without 700 nM exogenous calcium using saturation analysis and gel-filtration chromatography.
Separated bound from free ligand using perchlorate and hydroxylapatite methods, and analyzed lipid raft/caveolae-enriched fractions from rat and mouse intestine, ROS 17/2.8, and NB4 cells by Western blot.
Across 30 experiments, no specific binding of [3H]1,25(OH)2-D3 was observed with the annexin II monomer or heterotetramer, irrespective of 700 nM calcium presence.
Saturable [3H]1,25(OH)2-D3 binding occurred consistently in lipid raft/caveolae-enriched rat intestinal fractions (Kd = 3.0 nM; Bmax = 45 fmols/mg total protein), but exogenous calcium did not ablate binding.

Cite This Study

Mizwicki et al. (2004) studied this question.

synapsesocial.com/papers/6a8d0533c9fcfbd7f7906530 https://doi.org/10.1002/jcb.10783

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Implication

In vitro binding assays demonstrate lack of specific 1,25-vitamin D3 binding to annexin II, indicating annexin II functions as a downstream substrate rather than a direct membrane receptor.

Evidence that annexin II Is not a putative membrane receptor for 1α,25(OH)2‐vitamin D3

Discussion

Member takes

Implication

Key Points

Cite This Study

Evidence that annexin II Is not a putative membrane receptor for 1α,25(OH)2‐vitamin D3

Discussion

Member takes

Implication

Key Points

Cite This Study

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