Evidence for Loss of Lipid from Bovine Spermatozoa due to Freezing

The 1.26 mg/ml of lipid extracted from senfinal plasma separated from spermatozoa prior to freezing (prefreeze) was lower (P < 0.01) than the 1.92 mg/ml obtained from seminal plasma separated following freezing of the whole raw semen. There was also a significant difference (P ~ 0.05) between the 2.34 and 1.63 mg/ml extracted from the pre-and post-freeze spermatozoa. Freezing of whole raw semen prior to separation apparently results in an alteration in the quantity of lipid found in the cells and seminal plasma. Possible Mteration of lipid classes due to freezing was also observed.