Superoxide anions produce an endothelium-dependent contraction of isolated rabbit aorta that is partly mediated by an arachidonic acid metabolite.
Superoxide-driven endothelium-dependent contraction in rabbit aorta leaves open its role in human endothelial dysfunction; prospective studies needed before clinical translation.
We investigated the effects of superoxide anions (O 2 −) generated by xanthine (X) plus xanthine oxidase (XO) on isolated rabbit aorta suspended in Krebs-Ringer solution. Xanthine plus xanthine oxidase produced concentration-dependent contractions of rabbit aorta. Superoxide dismutase completely reversed the contractile response observed. Superoxide anion-induced contraction of rabbit aorta was totally abolished in preparations denuded of endothelium. The contractile effect was reduced by 56% by the cyclooxygenase inhibitor, indomethacin. The contractile effect was not affected by the angiotensin converting enzyme inhibitor, captopril. These results indicate that O 2 − produces a contraction of the isolated rabbit aorta that is endothelium-dependent and is partly mediated by an arachidonic acid metabolite.
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Bharadwaj et al. (2003) studied this question.
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