What question did this study set out to answer?

To determine whether parasympathetic cholinergic neurons of the embryonic chick ciliary ganglion express catecholaminergic traits in vivo and in vitro, and whether environmental signals influence this expression.

January 1, 1985Journal of NeuroscienceOpen Access

Cholinergic neurons of the chick ciliary ganglia express adrenergic traits in vivo and in vitro

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Authors

GTGladys TeitelmanSUNY Downstate Health Sciences University TJTH JohCornell University LGLindsay GraysonUnited States Fish and Wildlife Service

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Implication

Laboratory study demonstrates catecholaminergic enzyme expression in chick ciliary ganglion neurons, indicating environmental signals guide autonomic neurotransmitter plasticity.

Key Points

To determine whether parasympathetic cholinergic neurons of the embryonic chick ciliary ganglion express catecholaminergic traits in vivo and in vitro, and whether environmental signals influence this expression.
Examined embryonic day 8 (E8) chick ciliary ganglia for the catecholaminergic enzymes tyrosine hydroxylase (TH) and phenylethanolamine N-methyltransferase (PNMT) via immunocytochemistry.
Cultured dissociated E8 ciliary ganglion neurons for 3 to 12 days alone or co-cultured with embryonic notochord tissue.
Assessed aromatic L-amino acid decarboxylase (AADC) activity in cultured cells using levodopa incubation and catecholamine histofluorescence.
In vivo E8 ganglia contained rare TH-positive but no PNMT-positive neurons, whereas isolated cultures expressed TH or PNMT in 50% of culture dishes after 5 days.
Co-culturing ganglion neurons with notochord increased the proportion of dishes with TH- or PNMT-positive neurons to 90% after 5 days in vitro.
All neurons exhibited dopamine histofluorescence after 3 days of levodopa incubation regardless of notochord presence, indicating differential regulation of AADC relative to TH and PNMT.

Cite This Study

Teitelman et al. (1985) studied this question.

synapsesocial.com/papers/6a8d21140ee74fcafa4ac170 https://doi.org/10.1523/jneurosci.05-01-00029.1985

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Implication

Laboratory study demonstrates catecholaminergic enzyme expression in chick ciliary ganglion neurons, indicating environmental signals guide autonomic neurotransmitter plasticity.

Cholinergic neurons of the chick ciliary ganglia express adrenergic traits in vivo and in vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

Cholinergic neurons of the chick ciliary ganglia express adrenergic traits in vivo and in vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

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