What question did this study set out to answer?

To determine whether embryonic cerebral cortical neurons can express catecholaminergic phenotypes when grown in vitro and whether this capacity reflects phenotypic plasticity during development.

April 1, 1987Journal of NeuroscienceOpen Access

Expression of tyrosine hydroxylase in neurons of cultured cerebral cortex: evidence for phenotypic plasticity in neurons of the CNS

View Full Paper

Ask AI

Bookmark

Authors

LILorraine IacovittiThomas Jefferson University JLJin‐Moo LeeBiomax Informatics (Germany)TJTH JohCornell University

Discussion

Member takes

Implication

In vitro study demonstrates transient tyrosine hydroxylase expression in cultured embryonic rat cortical neurons, indicating developmental phenotypic plasticity.

Key Points

To determine whether embryonic cerebral cortical neurons can express catecholaminergic phenotypes when grown in vitro and whether this capacity reflects phenotypic plasticity during development.
Cultured embryonic rat cerebral cortex tissue obtained from donor embryos across embryonic days 13 through 18 (E13 to E18) for durations ranging from 1 to 14 days.
Evaluated tyrosine hydroxylase (TH) immunoreactivity and catalytic activity alongside neuron-specific enolase and 3H-thymidine incorporation to confirm neuronal identity and postmitotic status.
E13 cortical tissue cultured for 1 day yielded thousands of postmitotic neurons expressing catalytically active TH, whereas cortical neurons in vivo showed zero TH immunoreactivity.
The count of TH-expressing neurons in culture declined from nearly 3,000 cells at day 2 to several cells by day 14.
The capacity of cortical neurons to express TH in vitro diminished with advancing embryonic donor age, with very few neurons retaining competence by E18.

Cite This Study

Iacovitti et al. (1987) studied this question.

synapsesocial.com/papers/6a958fa32dd91e6efbf238d0 https://doi.org/10.1523/jneurosci.07-04-01264.1987

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates transient tyrosine hydroxylase expression in cultured embryonic rat cortical neurons, indicating developmental phenotypic plasticity.

Expression of tyrosine hydroxylase in neurons of cultured cerebral cortex: evidence for phenotypic plasticity in neurons of the CNS

Discussion

Member takes

Implication

Key Points

Cite This Study

Expression of tyrosine hydroxylase in neurons of cultured cerebral cortex: evidence for phenotypic plasticity in neurons of the CNS

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider