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Identify the environmental signal responsible for inducing expression of the catecholamine-synthesizing enzyme tyrosine hydroxylase in cultured embryonic rat cerebral cortical neurons.

October 1, 1989Journal of NeuroscienceOpen Access

A muscle-derived factor(s) induces expression of a catecholamine phenotype in neurons of cultured rat cerebral cortex

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Authors

LILorraine IacovittiThomas Jefferson University MEMJ EvingerStony Brook School TJTH JohCornell University

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Implication

In vitro study reveals that a muscle-derived factor induces catecholamine expression in cultured rat cortical neurons, suggesting environmental cues can drive central neuronal differentiation.

Key Points

Identify the environmental signal responsible for inducing expression of the catecholamine-synthesizing enzyme tyrosine hydroxylase in cultured embryonic rat cerebral cortical neurons.
Cultured embryonic rat cortical neurons with non-neuronal cells, blood vessels, perinatal muscle tissues, or clonal smooth (1440) and skeletal (L6) muscle cell lines.
Treated neuronal cultures with muscle cell-conditioned media, soluble L6 muscle extracts, macromolecular synthesis inhibitors (alpha-amanitin, cycloheximide), or known neurotrophic factors.
Quantified tyrosine hydroxylase (TH)-positive neurons via immunostaining, measured steady-state TH mRNA levels, and evaluated total neuronal survival.
Coculture with blood vessels increased TH-positive neurons nearly 4-fold, whereas coculture with muscle tissues, clonal muscle lines, or soluble muscle extracts increased TH-expressing neurons up to 20-fold.
Soluble muscle extract induced TH in a dose-dependent manner that correlated with increased TH mRNA and was abolished by alpha-amanitin or cycloheximide.
The phenotypic induction occurred without altering neuronal survival and was not mimicked by classic neurotrophic factors such as NGF, EGF, FGF, or PDGF.

Cite This Study

Iacovitti et al. (1989) studied this question.

synapsesocial.com/papers/6aa00d33b5fefa4cb5b57420 https://doi.org/10.1523/jneurosci.09-10-03529.1989

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Implication

In vitro study reveals that a muscle-derived factor induces catecholamine expression in cultured rat cortical neurons, suggesting environmental cues can drive central neuronal differentiation.

A muscle-derived factor(s) induces expression of a catecholamine phenotype in neurons of cultured rat cerebral cortex

Discussion

Member takes

Implication

Key Points

Cite This Study

A muscle-derived factor(s) induces expression of a catecholamine phenotype in neurons of cultured rat cerebral cortex

Discussion

Member takes

Implication

Key Points

Cite This Study

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