Identification of Shared Immune Cells and Immune-Related Co-Disease Genes in Chronic Heart Failure and Systemic Lupus Erythematosus Based on Transcriptome Sequencing

Figures

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  Figure 1. Screening of DEGs in HF and SLE. (A) Volcano plot showing DEGs in HF vs NC. (B) Heat map showing DEGs in HF vs NC. (C) Volcano plot showing DEGs in SLE vs NC. (D) Heat map showing DEGs in SLE vs NC. (E) Venn plot showing the intersection of DEGs in HF and SLE.

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  Figure 2. Results of functional enrichment analysis of HF and SLE. (A) Results of GSEA enrichment of all the genes in HF based on GO-BP. (B) Results of GSEA enrichment of all the genes in HF based on KEGG. (C) Results of GSEA enrichment of all the genes in SLE based on GO-BP. (D) Results of GSEA enrichment of all the genes in SLE based on KEGG. (E) GO-BP enrichment results of the common DEGs in HF and SLE. (F) GO-CC enrichment results of the common DEGs in HF and SLE. (G) GO-MF enrichment results of the common DEGs in HF and SLE. (H) KEGG enrichment results of the common DEGs in HF and SLE.

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  Figure 3. Immuno-infiltration analysis of HF and SLE. (A) Barplot showing immune cell expression in HF vs NC. (B) Correlation of immune cells in HF. (C) Violin plot showing the difference in immune cell expression in HF vs NC. (D) Barplot showing immune cell expression in SLE vs NC. (E) Correlation of immune cells in SLE. (F) Violin diagram demonstrating the difference in immune cell expression in SLE vs NC.

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  Figure 4. WGCNA of the shared immune cell-associated genes in HF. (A and B) Gene clustering dendrogram. (C) Scale-free fit indices for various soft threshold powers. (D) Average connectivity of soft threshold power functions. (E) Clustered dendrograms of co-expression network modules. (F) Heat map showing the correlation of module signature genes with the shared immune cells.

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  Figure 5. WGCNA of the shared immune cell-associated genes in SLE. (A and B) Gene clustering dendrograms. (C) Scale-free fit indices for various soft threshold powers. (D) Average connectivity of soft threshold power functions. (E) Clustered dendrograms of co-expression network modules. (F) Heat map showing the correlation of module signature genes with the shared immune cells.

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  Figure 6. Identification of the shared immune cell-related co-morbidity genes in HF and SLE. (A) Venn diagram showing the intersection of DEGs and T cell CD4 naïve-related genes common to HF and SLE. (B) PPI network of 207 intersected genes. (C) Upset plot showing intersected genes obtained by 5 algorithms in PPI analysis. (D) Venn diagram showing the intersection of DEGs and Monocytes-related genes common to HF and SLE. (E) PPI network of 162 intersected genes. (F) Upset plot showing the intersected genes obtained by 5 algorithms in PPI analysis.

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  Figure 7. Machine learning analysis. (A and B) Lasso analysis of T cells CD4 naïve related co-disease genes. (C) SVM analysis of T cells CD4 naïve-associated co-disease genes. (D and E) Random forest analysis of T cells CD4 naïve-associated co-disease genes. (F) Venn diagram showing the intersected genes obtained by 3 machine learning algorithms. (G and H) Lasso analysis of Monocytes-associated co-disease genes. (I) SVM analysis of Monocytes-associated co-disease genes. (J and K) Random forest analysis of Monocytes-associated co-disease genes. (L) Venn diagram showing the intersected genes obtained by 3 machine learning algorithms.

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  Figure 8. Validation of the differential expression of key genes between groups. (A–D) Box plots showing the expression of CCR7, RNASE2, RNASE3, and CXCL10 in HF vs NC. (E–H) Box plots showing the expression of CCR7, RNASE2, RNASE3, and CXCL10 in SLE vs NC.

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  Figure 9. Analysis of the diagnostic value of the key genes. (A–D) ROC analysis of the diagnostic value of CCR7, RNASE2, RNASE3, and CXCL10 in HF. (E–H) ROC analysis of the diagnostic value of CCR7, RNASE2, RNASE3, and CXCL10 in SLE.

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  Figure 10. Correlation analysis of the key genes with immune cells. (A–D) Lollipop plot showing the correlation between CCR7, RNASE2, RNASE3, and CXCL10 with immune cells in HF. (E–H) Lollipop plot demonstrating the correlation of CCR7, RNASE2, RNASE3, and CXCL10 with immune cells in SLE. (I) Correlation of CCR7, RNASE2, RNASE3, and CXCL10 in HF. (J) Correlation of CCR7, RNASE2, RNASE3, and CXCL10 in SLE.