June 30, 2006Journal of Biomedical Materials Research Part A
Mineralization of hydroxyapatite in electrospun nanofibrous poly( L ‐lactic acid) scaffolds
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Implication
Experimental study demonstrates enhanced hydroxyapatite mineralization on surface-hydrolyzed electrospun PLLA scaffolds, suggesting improved biomaterials for bone regeneration.
Key Points
- To investigate the deposition and growth of hydroxyapatite on electrospun poly(L-lactic acid) nanofibrous scaffolds, examining how surface functionalization and fluid additives influence mineralization.
- Fabricated porous poly(L-lactic acid) (PLLA) nanofibrous scaffolds via electrospinning and immersed them in simulated body fluids (SBF) at 37 °C.
- Modified scaffold surfaces using aqueous sodium hydroxide (NaOH) hydrolysis to introduce carboxylic acid groups, and tested the effects of anionic additives (citric acid and poly-L-aspartic acid) in the fluid.
- Characterized deposited minerals using scanning electron microscopy, energy-dispersive spectroscopy, wide-angle X-ray diffraction, Fourier transform infrared spectroscopy, and Raman spectroscopy.
- Mineralization produced a carbonated apatite mineral phase characterized by thin, flake-like nanostructures across the fiber surfaces.
- Inclusion of trace anionic additives (citric acid and poly-L-aspartic acid) in the simulated body fluid effectively inhibited mineral growth.
- Alkaline surface hydrolysis introduced carboxylate groups that bound calcium ions, significantly enhancing hydroxyapatite mineralization without compromising scaffold structural integrity.
Cite This Study
Chen et al. (2006) studied this question.