What question did this study set out to answer?

To determine the extent to which voltage-gated calcium channels contribute to the baseline intracellular calcium concentration in hippocampal CA1 pyramidal neurons.

November 1, 1996Journal of Neurophysiology

Dihydropyridine-sensitive, voltage-gated Ca2+ channels contribute to the resting intracellular Ca2+ concentration of hippocampal CA1 pyramidal neurons

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To determine the extent to which voltage-gated calcium channels contribute to the baseline intracellular calcium concentration in hippocampal CA1 pyramidal neurons.
Performed whole-cell recordings combined with high-speed ratiometric fluorescence imaging on hippocampal CA1 pyramidal neurons.
Assessed changes in somatic and apical dendritic intracellular calcium levels during sustained membrane hyperpolarization in the presence and absence of calcium channel antagonists (200 µM Cd2+, 10 µM nimodipine, and 50 µM Ni2+).
Membrane hyperpolarization provoked a sustained, voltage-dependent decline in intracellular calcium with a time constant of approximately 1 second, showing significantly higher baseline calcium at -50 mV than at -80 mV.
The hyperpolarization-evoked calcium drop was significantly attenuated by 200 µM Cd2+ and 10 µM nimodipine, while 50 µM Ni2+ produced only minor inhibition.
The greatest reduction in intracellular calcium occurred in the proximal apical dendrites, mirroring a slowly inactivating calcium current measured in acutely isolated neurons.

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Implication

Electrophysiological study demonstrates that dihydropyridine-sensitive calcium channels regulate resting intracellular calcium in hippocampal CA1 neurons, indicating tonic basal calcium entry.

Cite This Study

Magee et al. (1996) studied this question.

synapsesocial.com/papers/6a8d33bea4bc1cf4cf7aa92d https://doi.org/10.1152/jn.1996.76.5.3460

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Implication

Electrophysiological study demonstrates that dihydropyridine-sensitive calcium channels regulate resting intracellular calcium in hippocampal CA1 neurons, indicating tonic basal calcium entry.

Dihydropyridine-sensitive, voltage-gated Ca2+ channels contribute to the resting intracellular Ca2+ concentration of hippocampal CA1 pyramidal neurons

Key Points

Discussion

Implication

Cite This Study

Dihydropyridine-sensitive, voltage-gated Ca2+ channels contribute to the resting intracellular Ca2+ concentration of hippocampal CA1 pyramidal neurons

Key Points

Discussion

Implication

Cite This Study