Key result
A non-radioactive RT-PCR method allowed quantification of myosin heavy chain mRNA isoforms in rabbit muscles down to 1,000 molecules, revealing specific fast sequences for MHCIIb and MHCIId.
A non-radioactive RT-PCR method was successfully established to quantify myosin heavy chain mRNA isoforms in rabbit muscle tissue.
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May aid preclinical MHC isoform quantification in rabbits; leaves open human translation and clinical relevance.
Peuker et al. (1993) studied this question. Non-radioactive reverse transcriptase/polymerase chain reaction was evaluated on Quantification of myosin heavy chain mRNA isoforms. A non-radioactive RT-PCR method allowed quantification of myosin heavy chain mRNA isoforms in rabbit muscles down to 1,000 molecules, revealing specific fast sequences for MHCIIb and MHCIId.
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