Key result
LR White embedment maintained ultrastructural integrity but yielded a lower absolute signal than frozen sections, while antisense probe labeling averaged five times that of background.
LR White embedment provides superior ultrastructural preservation compared to frozen sections for electron microscopy in situ hybridization, making it suitable for detecting abundant messages like myosin heavy chain mRNA despite lower sensitivity.
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May support LR White for ultrastructure-preserving EM-ISH of abundant cardiac transcripts; leaves open sensitivity for low-abundance mRNAs.
Wenderoth et al. (1991) studied Normal cardiac tissue. LR White embedment vs. Frozen embedment (Ultracryomicrotomy) was evaluated on Ultrastructural integrity and content of endogenous mRNA. LR White embedment maintained ultrastructural integrity but yielded a lower absolute signal than frozen sections, while antisense probe labeling averaged five times that of background.
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