January 30, 2002Biochemistry

A Novel Restricted Photoaffinity Spin-Labeled Non-Nucleoside ATP Analogue as a Covalently Attached Reporter Group of the Active Site of Myosin Subfragment 1

View Full Paper

Ask AI

Bookmark

Population

Rabbit skeletal myosin subfragment 1 (S1)

Comparison

Photoaffinity spin-labeled non-nucleoside ATP… vs Parent compound NANTP (2-[amino]ethyl…

Design

Preclinical

Authors

XCXiaoru ChenAstraZeneca (United Kingdom)JGJean C. GrammerWashington State University JLJ. David LawsonPhusis Therapeutics (United States)

Discussion

Member takes

Implication

Reveals distinct ATP analogue orientations in skeletal myosin; leaves open translation to cardiac isoforms or targeted therapies.

Structured PICO

Population

Rabbit skeletal myosin subfragment 1 (S1)

Intervention

Photoaffinity spin-labeled non-nucleoside ATP analogue (SSL-NANTP)

Comparator

Parent compound NANTP (2-[(4-azido-2-nitrophenyl)amino]ethyl triphosphate)

Outcome

Site of photoincorporation in the nucleotide pocketsurrogate

This study provides the first direct evidence that Lys-681 in the 20 kDa tryptic fragment of skeletal myosin is close enough to the active site to be photolabeled by trapped ATP analogues.

Cite This Study

Chen et al. (2002) studied this question.

synapsesocial.com/papers/6a8e5ee2e3420198dacd63a2 https://doi.org/10.1021/bi0118411

View Full Paper

Ask AI

Bookmark

A Novel Restricted Photoaffinity Spin-Labeled Non-Nucleoside ATP Analogue as a Covalently Attached Reporter Group of the Active Site of Myosin Subfragment 1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A Novel Restricted Photoaffinity Spin-Labeled Non-Nucleoside ATP Analogue as a Covalently Attached Reporter Group of the Active Site of Myosin Subfragment 1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider